J*****o
About Candidate
Technical:
Programming & Data Analysis
• R: Advanced proficiency in building population models (growth, stochastic, species distribution), environmental and climate data analysis, and generating high-quality data visualizations
• Python: Experience developing a functional web tool for oligonucleotide search, including code debugging and structural design
Laboratory Quantification & Analysis
• Skilled in quantitative analysis of Western blots, Northern blots, and native blots
• Experienced in cell metabolism quantification and data interpretation
Language: Chinese: Native English: High proficient
Laboratory:
Western Blot,Northern Blot,Native Blot,Human induced-pluripotent cells culture, ELISA, PCR, Cell Viability Assay, Quantification & Analysis High-throughput Screening
Salary
Nationality
Looking for Job Title
Iqama
Location
Education
BS in Computational Biology | Cluster in Bioethics & History of Science, Technology, and Medicine Current GPA: 3.81/4.0, expected to graduate in December 2026. Relevant Coursework: tRNA Modification & Disease Research, Ecological Data Analysis, Conservation Genetics & Modeling, Biochemistry, Organic Chemistry, Discrete Math, Multivariable Calculus Scholarship: Kearns ’Scholar, Schwartz Discover Grant Recipient, Dean’s list
Work & Experience
• Investigated cytoplasmic and mitochondrial tRNA charging defects resulting from TRMT1 loss using acidic northern blotting and native gel assays • Quantified mt-tRNA^Ile aminoacylation deficits and validated rescue via TRMT1 re-expression in HEK293T knockout models • Assessed TRMT1 variant effects on tRNA structure and m^2,2G modification using patient-derived fibroblasts and structural probing assays • Evaluated cell proliferation phenotypes in TRMT1-deficient human fibroblasts and mouse embryonic fibroblasts via longitudinal viability assays • Integrated clinical variant data with molecular phenotypes to identify patient-specific TRMT1 dysfunction linked to neurodevelopmental disorder • Culture wildtype and tRNA-methyltransferase-1 (TRMT1) gene knock out iPSCs and cerebral organoids to investigate how TRMT1 mutations impact to neural development across differentiation stages. • Perform 3D imaging and quantitative analysis of brain organoids using Confocal Microscopy and Light-Sheet Fluorescence Microscopy, identifying structural and functional changes linked to TRMT1 loss-of-function. • Collaborated with multidisciplinary teams across UCSC, MIT, SUNY Albany, NTNU, and University of Munich to contextualize mechanistic findings
• Analyzed retinal vascular density and permeability using MRI contrast imaging to identify neovascularization patterns associated with angiogenesis progression. • Developed an AI-based image analysis pipeline to automatically identify and segment retinal blood vessels and lesions, including microaneurysms and neovascular tufts • Optimized the retina staining protocol to increase consistency and clarity of experimental results; Standardized preparation and handling procedures to ensure accuracy, safety, and reproducibility in the lab
• Supported daily laboratory operations, including equipment upkeep, material restocking, and supply ordering • Maintained and calibrated lab instruments to ensure smooth and reliable operation • Monitored and regulated gas systems and pressure levels to maintain safe and stable laboratory conditions • Assisted with troubleshooting technical issues and coordinating repairs to minimize experiment downtime • Organized workspaces and inventories to improve lab efficiency and compliance with safety standards
